DNA repair after CRISPR cutting not at all what people thought

Scientists discovered that a well-known DNA repair pathway, the Fanconi anemia pathway, surprisingly plays a key role in repairing double-strand DNA breaks created by CRISPR-Cas9. It acts as a traffic cop to steer repair to simple end-joining or to patching the cut with new, single-strand DNA. Scientists could potentially tweak proteins involved in the pathway to preferentially steer the outcome toward replacement with DNA, which is important for gene therapy for hereditary diseases. (Mehr in: Cancer News — ScienceDaily)