A team of biophysicists led by Prof. Dr. Oliver Bäumchen at the University of Bayreuth and biologists led by Dr. Maike Lorenz at the Culture Collection of Algae at Göttingen University has published a step-by-step guide for the reliable cultivation of the unicellular green alga Chlamydomonas reinhardtii. The guide will support researchers worldwide in the fields of life sciences, biophysics, and bioengineering in their investigations of biological, biophysical, and biotechnological principles. Their reliable cultivation methods are detailed in Nature Protocols.