Tuberculosis is one of the leading causes of death worldwide, with an estimated 1.4 million deaths and ten million people infected annually. Resistant and multidrug-resistant (MDR) variants of the tuberculosis pathogen Mycobacterium tuberculosis pose a major threat to tuberculosis control and global health. Rapid detection of these patient-specific resistance patterns is therefore crucial for targeted treatment and successful control of the transmission of antibiotic-resistant tuberculosis bacteria—a goal that DZIF scientists have now taken a major step towards.