Generation of mouse induced pluripotent stem cells succeeds better without Oct4

Quality of induced pluripotent stem cells is dramatically enhanced by omitting what was thought to be the most crucial reprogramming factor

Scientists at the Max Planck Institute for Molecular Biomedicine in Münster showed that Oct4, a factor most commonly used for reprogramming, is detrimental for the quality of induced pluripotent stem cells (iPSCs). Omitting Oct4 from the reprogramming cocktail allowed for the generation of mouse iPSCs with the highest developmental potential reported to date (Cell Stem Cell, November 7, 2019, epub ahead of print).

Quelle: IDW Informationsdienst Wissenschaft