One for all: super-resolution microscopy with one universal nanobody

To make cellular structures easily accessible for super-resolution microscopy, scientists at the Max Planck Institute for Terrestrial Microbiology in Marburg, the Natural and Medical Sciences Institute (NMI) in Reutlingen and the Eberhard Karls University of Tübingen have developed a new strategy for dense, unobtrusive labeling of proteins. In a study now published in Nature Communications, the authors utilize an inert peptide-tag in combination with a high affinity nanobody for close-grained fluorophore tagging with minimal linkage errors. The authors expect that this approach will now become broadly applicable to visualize multiple cellular targets in super-resolution microscopy. (Mehr in: Pressemitteilungen – idw – Informationsdienst Wissenschaft)